Streptococcus mutans를 억제하는 오배자 구성물질의 분리 및 정제에 관한 연구
Identification and partial purification of antibacterial compounds against Streptococcus mutans from Galla Rhois
신애리, 옥승호, 최충호, 홍석진,
소속 상세정보
신애리 ( Shin Ae-Ri ) - 전남대학교 치의학전문대학원 예방치과학교실
옥승호 ( Ohk Seung-Ho ) - 전남대학교 치의학전문대학원 구강미생물학교실
최충호 ( Choi Choong-Ho ) - 전남대학교 치의학전문대학원 예방치과학교실
홍석진 ( Hong Suk-Jin ) - 전남대학교 치의학전문대학원 예방치과학교실
KMID : 0355420160400010003
Abstract
Objectives: This study aims to identify and partially purify antibacterial compounds against Streptococ-cus mutans from Galla Rhois extract.
Methods: Galla Rhois was extracted with n-hexane or ethanol and concentrated in a rotary evaporator. The antibacterial effect of the Galla Rhois extract against S. mutans was determined by the paper disc-diffusion method with n-hexane, ethanol, methanol, ethyl acetate, dimethyl sulfoxide (DMSO), acetone, and distilled water as the solvents. The active compounds were purified by partition chromatography, thin-layer chromatography (TLC), and high-performance liquid chromatography (HPLC).
Results: The antibacterial effect of the n-hexane extract was more effective against S. mutans than the ethanol extract (P<0.05). The antibacterial component of Galla Rhois was partially purified using parti-tion chromatography and HPLC, and the antibacterial activity was confirmed.
Conclusions: The partially purified component of Galla Rhois showed strong antibacterial effect against S. mutans. These results confirm that the antibacterial compounds of Galla Rhois can be used for the prevention of dental caries.
키워드
Antibacterial compounds; Column chromatography; Galla Rhois; Partition chromatogra-phy; Streptococcus mutans; Thin-layer chromatography
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